The foreign ministry said neither India nor Iran needed external guidance on how to conduct bilateral relations.
It said relations between the two spanned centuries, and they were capable of handling them with due care.
Earlier, a senior US official said Washington would welcome India telling Iranian President Mahmoud Ahmadinejad to curtail Tehran's nuclear programme.
President Ahmadinejad is due to visit Delhi next week.
"India and Iran are ancient civilisations whose relations span centuries. Both nations are perfectly capable of managing all aspects of their relationship with the appropriate degree of care and attention," Navtej Sarna, a foreign ministry spokesman, was quoted by the Indian Express newspaper as saying.
"Neither country needs any guidance on the future conduct of bilateral relations as both countries believe that engagement and dialogue alone lead to peace," he added.
Somewhere, Ayaz Amir just had an aneurysm.